Article
Sequence-verified two-allele transposon mutant library for Pseudomonas aeruginosa PAO1.
Journal of bacteriology - 1 Dec 2012
Held Kiara, Ramage Elizabeth, Jacobs Michael, Gallagher Larry, Manoil Colin
Abstract excerpt
Mutant hunts using comprehensive sequence-defined libraries make it possible to identify virtually all of the nonessential functions required for different bacterial processes. However, the success of such screening depends on the accuracy of mutant identification in the mutant library used. To provide a high-quality library for Pseudomonas aeruginosa PAO1, we created a sequence-verified collection of 9,437...
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