Article
An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants.
Proceedings of the National Academy of Sciences of the United States of America - 21 Feb 2006
Liberati Nicole T, Urbach Jonathan M, Miyata Sachiko, Lee Daniel G, Drenkard Eliana, Wu Gang, Villanueva Jacinto, Wei Tao, Ausubel Frederick M
Abstract excerpt
Random transposon insertion libraries have proven invaluable in studying bacterial genomes. Libraries that approach saturation must be large, with multiple insertions per gene, making comprehensive genome-wide scanning difficult. To facilitate genome-scale study of the opportunistic human pathogen Pseudomonas aeruginosa strain PA14, we constructed a nonredundant library of PA14 transposon mutants (the PA14NR Set)...
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