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ORBIT: a New Paradigm for Genetic Engineering of Mycobacterial Chromosomes

10 Dec 2018

Abstract excerpt

We sought to develop a system that could increase the usefulness of oligonucleotide-mediated recombineering of bacterial chromosomes by expanding the types of modifications generated by an oligonucleotide (i.e., insertions and deletions) and by making recombinant formation a selectable event. This paper describes such a system for use in M. smegmatis and M. tuberculosis . By incorporating a single-stranded DNA...

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PMID: 30538179
DOI: 10.1128/mbio.01467-18

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