Article
Phage-assisted continuous evolution of enzymes for noncanonical tyrosine biosynthesis.
Proceedings of the National Academy of Sciences of the United States of America - 15 Sept 2026
Andon James S, Behera Abhijit, Deb Debashrito, Weeks Amy M, Buller Andrew R, Wang Tina
Abstract excerpt
Genetic code expansion introduces new-to-nature chemical moieties into ribosomally synthesized proteins. In practice, the scope of functional groups that can be accessed using this method is often limited by noncanonical amino acid (ncAA) availability. Producing ncAAs directly in cells can circumvent poor ncAA uptake or commercial unavailability, but limited enzymes suitable for this application exist. In vitro...
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- Tyrosine
- Escherichia coli
- Directed Molecular Evolution
- Bacteriophages
- Mutation