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Article

A new method to accurately identify single nucleotide variants using small FFPE breast samples.

Briefings in bioinformatics - 5 Nov 2021

Fortunato Angelo, Mallo Diego, Rupp Shawn M, King Lorraine M, Hardman Timothy, Lo Joseph Y, Hall Allison, Marks Jeffrey R, Hwang E Shelley, Maley Carlo C

Abstract excerpt

Most tissue collections of neoplasms are composed of formalin-fixed and paraffin-embedded (FFPE) excised tumor samples used for routine diagnostics. DNA sequencing is becoming increasingly important in cancer research and clinical management; however it is difficult to accurately sequence DNA from FFPE samples. We developed and validated a new bioinformatic pipeline to use existing variant-calling strategies to...

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Identifiers

PMID: 34117742
DOI: 10.1093/bib/bbab221
PMCID: PMC8574974

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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