Article
Robust gene expression and mutation analyses of RNA-sequencing of formalin-fixed diagnostic tumor samples.
Scientific reports - 23 Jul 2015
Graw Stefan, Meier Richard, Minn Kay, Bloomer Clark, Godwin Andrew K, Fridley Brooke, Vlad Anda, Beyerlein Peter, Chien Jeremy
Abstract excerpt
Current genomic studies are limited by the availability of fresh tissue samples. Here, we show that Illumina RNA sequencing of formalin-fixed diagnostic tumor samples produces gene expression that is strongly correlated with matched frozen tumor samples (r > 0.89). In addition, sequence variations identified from FFPE RNA show 99.67% concordance with that from exome sequencing of matched frozen tumor samples....
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