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Article

Comparison of clinical targeted next-generation sequence data from formalin-fixed and fresh-frozen tissue specimens.

The Journal of molecular diagnostics : JMD - 1 Sept 2013

Spencer David H, Sehn Jennifer K, Abel Haley J, Watson Mark A, Pfeifer John D, Duncavage Eric J

Abstract excerpt

Next-generation sequencing (NGS) has emerged as a powerful technique for the detection of genetic variants in the clinical laboratory. NGS can be performed using DNA from FFPE tissue, but it is unknown whether such specimens are truly equivalent to unfixed tissue for NGS applications. To address this question, we performed hybridization-capture enrichment and multiplexed Illumina NGS for 27 cancer-related genes...

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Identifiers

PMID: 23810758
DOI: 10.1016/j.jmoldx.2013.05.004
PMCID: PMC4912568

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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