Article
Identification of high-confidence somatic mutations in whole genome sequence of formalin-fixed breast cancer specimens.
Nucleic acids research - 1 Aug 2012
Yost Shawn E, Smith Erin N, Schwab Richard B, Bao Lei, Jung HyunChul, Wang Xiaoyun, Voest Emile, Pierce John P, Messer Karen, Parker Barbara A, Harismendy Olivier, Frazer Kelly A
Abstract excerpt
The utilization of archived, formalin-fixed paraffin-embedded (FFPE) tumor samples for massive parallel sequencing has been challenging due to DNA damage and contamination with normal stroma. Here, we perform whole genome sequencing of DNA isolated from two triple-negative breast cancer tumors archived for >11 years as 5 µm FFPE sections and matched germline DNA. The tumor samples show differing amounts of FFPE...
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