Refined variant calling pipeline on RNA-seq data of breast cancer cell lines without matched-normal samples.

Abstract excerpt

OBJECTIVE: RNA-seq delivers valuable insights both to transcriptional patterns and mutational landscapes for transcribed genes. However, as tumour cell lines frequently lack their matched-normal counterpart, variant calling without the paired normal sample is still challenging. In order to exclude variants of common genetic variation without a matched-normal control, filtering strategies need to be developed to...

Topics

• Humans
• Breast Neoplasms
• Cell Line, Tumor
• Female
• RNA-Seq
• Polymorphism, Single Nucleotide
• Genetic Variation