Article
Accurate genomic variant detection in single cells with primary template-directed amplification.
Proceedings of the National Academy of Sciences of the United States of America - 15 Jun 2021
Gonzalez-Pena Veronica, Natarajan Sivaraman, Xia Yuntao, Klein David, Carter Robert, Pang Yakun, Shaner Bridget, Annu Kavya, Putnam Daniel, Chen Wenan, Connelly Jon, Pruett-Miller Shondra, Chen Xiang, Easton John, Gawad Charles
Abstract excerpt
Improvements in whole genome amplification (WGA) would enable new types of basic and applied biomedical research, including studies of intratissue genetic diversity that require more accurate single-cell genotyping. Here, we present primary template-directed amplification (PTA), an isothermal WGA method that reproducibly captures >95% of the genomes of single cells in a more uniform and accurate manner than...
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