Article
Use of the rpoB gene to determine the specificity of base substitution mutations on the Escherichia coli chromosome.
DNA repair - 13 May 2003
Garibyan Lilit, Huang Tiffany, Kim Mandy, Wolff Erika, Nguyen Anh, Nguyen Theresa, Diep Amy, Hu Kaibin, Iverson Ayuko, Yang Hanjing, Miller Jeffrey H
Abstract excerpt
Mutations in the rpoB gene of Escherichia coli result in resistance to the antibiotic rifampicin (Rif(r)) by altering the beta subunit of RNA polymerase. Previous studies have identified 39 single base substitutions in the rpoB gene that lead to Rif(r) at 37 degrees C and an additional two mutations that result in temperature sensitive cells. We have extended this work and identified an additional 30 single base...
Topics
- 2-Aminopurine
- Adenosine Triphosphatases
- Antibiotics, Antitubercular
- Azacitidine
- Bacterial Proteins
- Binding, Competitive
- Chromosomes
- Cisplatin
- Cross-Linking Reagents
- DNA Repair
- DNA-Binding Proteins
- DNA-Directed RNA Polymerases
- Drug Resistance, Bacterial