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Article

Substrate Phosphorylation Rates as an In Vivo Measurement of Kinase Activity.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Swaffer Matthew P

Abstract excerpt

Measuring kinase activity in different in vivo contexts is crucial for understanding the mechanism and functions of protein kinases, such as the cyclin-dependent kinases (Cdks) and other cell cycle kinases. Here, I present the rationale and the experimental framework for an alternative approach to measure kinase activity that is based on estimating substrate phosphorylation rates in vivo. The approach presented...

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Identifiers

PMID: 34085212
DOI: 10.1007/978-1-0716-1538-6_2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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