Use of phospho-site substitutions to analyze the biological relevance of phosphorylation events in regulatory networks.

Abstract excerpt

Biological information is often transmitted by phosphorylation cascades. However, the biological relevance of specific phosphorylation events is often difficult to determine. An invaluable tool to study the effect of kinases and/or phosphatases is the use of phospho- and dephospho-mimetic substitutions in the respective target proteins. Here, we present a generally applicable procedure of how to design, set-up,...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Blotting, Western
• Share to Cell Cycle
• Share to Chromatography, Affinity