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Article

Efficient CRISPR/Cas9 mutagenesis for neurobehavioral screening in adult zebrafish.

G3 (Bethesda, Md.) - 7 Aug 2021

Klatt Shaw Dana, Mokalled Mayssa H

Abstract excerpt

Adult zebrafish are widely used to interrogate mechanisms of disease development and tissue regeneration. Yet, the prospect of large-scale genetics in adult zebrafish has traditionally faced a host of biological and technical challenges, including inaccessibility of adult tissues to high-throughput phenotyping and the spatial and technical demands of adult husbandry. Here, we describe an experimental pipeline...

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Identifiers

PMID: 33742663
DOI: 10.1093/g3journal/jkab089
PMCID: PMC8496216

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