Article
Rapid Testing of Gene Function in Axonal Regeneration After Spinal Cord Injury Using Larval Zebrafish.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2023
Drake Louisa K, Keatinge Marcus, Tsarouchas Themistoklis M, Becker Catherina G, Lyons David A, Becker Thomas
Abstract excerpt
Larval zebrafish show axonal regrowth over a complex spinal injury site and recovery of function within days after injury. Here we describe a simple protocol to disrupt gene function in this model using acute injections of highly active synthetic gRNAs to rapidly detect loss-of-function phenotypes without the need for breeding.
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.