DiRect: Site-directed mutagenesis method for protein engineering by rational design.

Abstract excerpt

Site-directed mutagenesis (SDM), an indispensable method in molecular biology and protein engineering, is rather time-consuming and laborious. Protein engineering, especially that of enzymes, nowadays increasingly relies on rational design approaches in which both SDM and protein expression are the bottlenecks because they are generally based on the recombinant DNA technology. Here, we developed a new PCR-based...

Topics

Join the communities discussing this publication.

• Cell-Free System
• Enzyme Stability
• Mutagenesis, Site-Directed
• Mutation
• NADP