Article
Enzyme improvement in the absence of structural knowledge: a novel statistical approach.
The ISME journal - 1 Feb 2008
Barak Yoram, Nov Yuval, Ackerley David F, Matin A
Abstract excerpt
Most existing methods for improving protein activity are laborious and costly, as they either require knowledge of protein structure or involve expression and screening of a vast number of protein mutants. We describe here a successful first application of a novel approach, which requires no structural knowledge and is shown to significantly reduce the number of mutants that need to be screened. In the first...
Topics
- Biotechnology
- Chromates
- Computational Biology
- Directed Molecular Evolution
- Escherichia coli
- Mutagenesis, Site-Directed
- Mutation
- NADP
- Oxidation-Reduction
- Oxidoreductases
- Prodrugs