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Article

Improved EGFR mutation detection sensitivity after enrichment by Cas9/sgRNA digestion and PCR amplification.

Acta biochimica et biophysica Sinica - 29 Dec 2020

Wang Li, Jiang Jing, Li Xiangning, Li Kai, He Rongzhang, Li Jia, Duan Lili, Luo Weihao, Hu Zheng, Luo Dixian

Abstract excerpt

The detection of circulating tumor DNA is important in cancer research and clinical practice. In the present study, we aimed to improve the sensitivity of downstream mutation detection of next-generation sequencing using the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) system to selectively target wild-type fragments but with low or no cleavage activity to...

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PMID: 33216845
DOI: 10.1093/abbs/gmaa123

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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