Article
Evaluation of CRISPR/Cas9 site-specific function and validation of sgRNA sequence by a Cas9/sgRNA-assisted reverse PCR technique.
Analytical and bioanalytical chemistry - 1 Apr 2021
Zhang Beibei, Zhou Jiamu, Li Miao, Wei Yuanmeng, Wang Jiaojiao, Wang Yange, Shi Pingling, Li Xiaoli, Huang Zixu, Tang He, Song Zongming
Abstract excerpt
The effective application of the clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system in biology, medicine and other fields is hindered by the off-target effects and loci-affinity of Cas9-sgRNA, especially at a genome-wide scale. In order to eliminate the occurrence of of...
Topics
- Base Sequence
- CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats
- DNA
- DNA Mutational Analysis
- Gene Editing
- Mutation
- Polymerase Chain Reaction
- RNA, Guide, CRISPR-Cas Systems