Article
Highly specific detection of KRAS single nucleotide polymorphism by asymmetric PCR/SERS assay.
The Analyst - 13 Sept 2021
Lyu Nana, Rajendran Vinoth Kumar, Li Jun, Engel Alexander, Molloy Mark P, Wang Yuling
Abstract excerpt
The molecular diagnosis of KRAS mutations has become crucial for clinical decision-making in colorectal cancer (CRC) treatments. Currently, the common methods for detecting mutations are based on quantitative PCR, DNA sequencing and droplet digital PCR (ddPCR), which require expensive specialized equipment and testing reagents. Herein, we propose a simple and specific strategy by integrating asymmetric PCR with...
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