Article
Single-Tubed Wild-Type Blocking Quantitative PCR Detection Assay for the Sensitive Detection of Codon 12 and 13 KRAS Mutations.
PloS one - 1 Jan 2015
Huang Jun-Fu, Zeng Dong-Zhu, Duan Guang-Jie, Shi Yan, Deng Guo-Hong, Xia Han, Xu Han-Qing, Zhao Na, Fu Wei-Ling, Huang Qing
Abstract excerpt
The high degree of intra-tumor heterogeneity has meant that it is important to develop sensitive and selective assays to detect low-abundance KRAS mutations in metastatic colorectal carcinoma (mCRC) patients. As a major potential source of tumor DNA in the aforementioned genotyping assays, it was necessary to conduct an analysis on both the quality and quantity of DNA extracted from formalin-fixed...
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