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Article

Functional analysis of the catalytic triad of the hAT-family transposase TcBuster.

Plasmid - 1 Mar 2021

Woodard Lauren E, Williams Felisha M, Jarrett Isria C, Wilson Matthew H

Abstract excerpt

TcBuster is a hAT-family DNA transposon from the red flour beetle, Tribolium castaneum. The TcBuster transposase is of interest for genome engineering as it is highly active in insect and mammalian cells. To test the predicted catalytic triad of TcBuster, each residue of the catalytic triad of a haemagglutinin-tagged TcBuster transposase was individually mutated to a structurally conserved amino acid. Using a...

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Identifiers

PMID: 33476638
DOI: 10.1016/j.plasmid.2021.102554
PMCID: PMC7946762

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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