Article
A method for characterizing Cas9 variants via a one-million target sequence library of self-targeting sgRNAs.
Nucleic acids research - 6 Apr 2021
Tálas András, Huszár Krisztina, Kulcsár Péter István, Varga Julia K, Varga Éva, Tóth Eszter, Welker Zsombor, Erdős Gergely, Pach Péter Ferenc, Welker Ágnes, Györgypál Zoltán, Tusnády Gábor E, Welker Ervin
Abstract excerpt
Detailed target-selectivity information and experiment-based efficacy prediction tools are primarily available for Streptococcus pyogenes Cas9 (SpCas9). One obstacle to develop such tools is the rarity of accurate data. Here, we report a method termed 'Self-targeting sgRNA Library Screen' (SLS) for assaying the activity of Cas9 nucleases in bacteria using random target/sgRNA libraries of self-targeting sgRNAs....
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