Article
Alternative RNA degradation pathways by the exonuclease Pop2p from Saccharomyces cerevisiae.
RNA (New York, N.Y.) - 1 Apr 2021
Ye Xuan, Axhemi Armend, Jankowsky Eckhard
Abstract excerpt
The 3' to 5' exonuclease Pop2p (Caf1p) is part of the CCR4-NOT deadenylation complex that removes poly(A) tails from mRNAs in cells. Pop2p is structurally conserved in eukaryotes, but Saccharomyces cerevisiae Pop2p harbors noncanonical amino acids in its catalytic center. The enzymatic properties of S. cerevisiae Pop2p are not well defined. Here we characterize the RNA exonuclease activity of recombinant S....
Topics
- Adenosine Monophosphate
- Amino Acid Substitution
- Amino Acids
- Binding Sites
- Catalytic Domain
- Cloning, Molecular
- Escherichia coli
- Gene Expression
- Genetic Vectors
- Models, Molecular
- Mutation