Article
A simplified strategy for titrating gene expression reveals new relationships between genotype, environment, and bacterial growth.
Nucleic acids research - 11 Jan 2021
Mathis Andrew D, Otto Ryan M, Reynolds Kimberly A
Abstract excerpt
A lack of high-throughput techniques for making titrated, gene-specific changes in expression limits our understanding of the relationship between gene expression and cell phenotype. Here, we present a generalizable approach for quantifying growth rate as a function of titrated changes in gene expression level. The approach works by performing CRISPRi with a series of mutated single guide RNAs (sgRNAs) that...
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