Article
Construction of Escherichia coli gene expression level perturbation collection.
Metabolic engineering - 1 Jan 2009
Kashiwagi Akiko, Sakurai Takahiro, Tsuru Saburo, Ying Bei-Wen, Mori Kotaro, Yomo Tetsuya
Abstract excerpt
We generated 61 strains of Escherichia coli in which the expression level of a specific single gene can be changed continuously over a physiologically significant range. In each strain, one auxotrophic gene was deleted from its original position and reinserted at a specific position on the chromo...
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