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Article

Extensive trimming of short single-stranded DNA oligonucleotides during replication-coupled gene editing in mammalian cells.

PLoS genetics - 1 Oct 2020

van Ravesteyn Thomas W, Arranz Dols Marcos, Pieters Wietske, Dekker Marleen, Te Riele Hein

Abstract excerpt

Through transfection of short single-stranded oligodeoxyribonucleotides (ssODNs) small genomic alterations can be introduced into mammalian cells with high precision. ssODNs integrate into the genome during DNA replication, but the resulting heteroduplex is prone to detection by DNA mismatch repair (MMR), which prevents effective gene modification. We have previously demonstrated that the suppressive action of...

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Identifiers

PMID: 33119594
DOI: 10.1371/journal.pgen.1009041
PMCID: PMC7595315

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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