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Article

Bottom-up single-molecule strategy for understanding subunit function of tetrameric β-galactosidase.

Proceedings of the National Academy of Sciences of the United States of America - 14 Aug 2018

Li Xiang, Jiang Yu, Chong Shaorong, Walt David R

Abstract excerpt

In this paper, we report an example of the engineered expression of tetrameric β-galactosidase (β-gal) containing varying numbers of active monomers. Specifically, by combining wild-type and single-nucleotide polymorphism plasmids at varying ratios, tetrameric β-gal was expressed in vitro with one to four active monomers. The kinetics of individual enzyme molecules revealed four distinct populations,...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 30061400
DOI: 10.1073/pnas.1805690115
PMCID: PMC6099853

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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