Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Design of an alternate antibody fragment format that can be produced in the cytoplasm of Escherichia coli

30 Aug 2023

Abstract excerpt

Abstract With increased accessibility and tissue penetration, smaller antibody formats such as antibody fragments (Fab) and single chain variable fragments (scFv) show potential as effective and low-cost choices to full-length antibodies. These formats derived from the modular architecture of antibodies could prove to be game changers for certain therapeutic and diagnostic applications. Microbial hosts have shown...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Bacteriophages and microbial interactions
Share to Monoclonal and Polyclonal Antibodies Research
Share to Protein purification and stability

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Source

Identifiers

PMID: 37648872
DOI: 10.1038/s41598-023-41525-3

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 23 Oct 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Design of an alternate antibody fragment format that can be produced in the cytoplasm of Escherichia coliPMID 37648872Read ↗

Bacterial cytoplasm as an effective cell compartment for producing functional VHH-based affinity reagents and Camelidae IgG-like recombinant antibodiesSemantic proximity Read ↗

Solubility, Stability, and Avidity of Recombinant Antibody Fragments Expressed in MicroorganismsSemantic proximity Read ↗

Fab2-SARAH: An Escherichia coli-Based Antibody Fragment Platform for Scalable, Cost-Effective In Vitro DiagnosticsSemantic proximity Read ↗

Challenges in Expression and Purification of Functional Fab Fragments in E. coli: Current Strategies and PerspectivesSemantic proximity Read ↗

Streamlining the Transition From Yeast Surface Display of Antibody Fragment Immune Libraries to the Production as IgG Format in Mammalian CellsSemantic proximity Read ↗

Efficient expression of full-length antibodies in the cytoplasm of engineered bacteriaSemantic proximity Read ↗

Protein engineering of antibody fragments for pharmaceutical productionSemantic proximity Read ↗

Antibody Production Techniques and Antibody Types: Evolution, Methodologies, and ApplicationsSemantic proximity Read ↗

Optimizing antibody expression by using the naturally occurring framework diversity in a live bacterial antibody display system.Semantic proximity Read ↗

Functional expression and display of an antibody Fab fragment in Escherichia coli: study of vector designs and culture conditionsSemantic proximity Read ↗

A multi-Fc-species system for recombinant antibody productionSemantic proximity Read ↗

Rapid screening platform for stabilization of scFvs in Escherichia coli.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.