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Article

Optimizing antibody expression by using the naturally occurring framework diversity in a live bacterial antibody display system.

Scientific reports - 3 Dec 2015

Lombana T Noelle, Dillon Michael, Bevers Jack, Spiess Christoph

Abstract excerpt

Rapid identification of residues that influence antibody expression and thermostability is often needed to move promising therapeutics into the clinic. To establish a method that can assess small expression differences, we developed a Bacterial Antibody Display (BAD) system that overcomes previous limitations, enabling the use of full-length formats for antibody and antigen in a live cell setting. We designed a...

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Antibodies
Escherichia coli
Interleukin-13
Peptide Library
Protein Engineering

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Stability

Recombinant Proteins

Vascular Endothelial Growth Factor A

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Open in PubMed Open full text

Identifiers

PMID: 26631978
DOI: 10.1038/srep17488
PMCID: PMC4668361

Genes

BAD

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

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