Article
Unbiased PCR-free spatio-temporal mapping of the mtDNA mutation spectrum reveals brain region-specific responses to replication instability.
BMC biology - 23 Oct 2020
Bagge Emilie Kristine, Fujimori-Tonou Noriko, Kubota-Sakashita Mie, Kasahara Takaoki, Kato Tadafumi
Abstract excerpt
BACKGROUND: The accumulation of mtDNA mutations in different tissues from various mouse models has been widely studied especially in the context of mtDNA mutation-driven ageing but has been confounded by the inherent limitations of the most widely used approaches. By implementing a method to sequence mtDNA without PCR amplification prior to library preparation, we map the full unbiased mtDNA mutation spectrum...
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