Article
Identification of EGFR and RAS Inhibitors using Caenorhabditis elegans.
Journal of visualized experiments : JoVE - 5 Oct 2020
van der Hoeven Dharini, Truong Thuy Nhu L, Naji Ali, Thapa Sabita, Hancock John F, van der Hoeven Ransome
Abstract excerpt
The changes in the plasma membrane localization of the epidermal growth factor receptor (EGFR) and its downstream effector RAS have been implicated in several diseases including cancer. The free-living nematode C. elegans possesses an evolutionary and functionally conserved EGFR-RAS-ERK MAP signal cascade which is central for the development of the vulva. Gain of function mutations in RAS homolog LET-60 and EGFR...
Topics
- Animals
- Caenorhabditis elegans
- Cell Membrane
- Drug Evaluation, Preclinical
- ErbB Receptors
- Female
- Humans
- Phenotype
- Protein Kinase Inhibitors
- Signal Transduction
- ras Proteins