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Article

Effective Blocking of Microbial Transcriptional Initiation by dCas9-NG-Mediated CRISPR Interference.

Journal of microbiology and biotechnology - 28 Dec 2020

Kim Bumjoon, Kim Hyun Ju, Lee Sang Jun

Abstract excerpt

CRISPR interference (CRISPRi) has been developed as a transcriptional control tool by inactivating the DNA cleavage ability of Cas9 nucleases to produce dCas9 (deactivated Cas9), and leaving dCas9 the ability to specifically bind to the target DNA sequence. CRISPR/Cas9 technology has limitations in designing target-specific single-guide RNA (sgRNA) due to the dependence of protospacer adjacent motif (PAM)...

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Identifiers

PMID: 32958732
DOI: 10.4014/jmb.2008.08058
PMCID: PMC9728369

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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