Article
In vitro affinity maturation to improve the efficacy of a hypoxia-inducible factor 1α single-domain intrabody.
Biochemical and biophysical research communications - 3 Sept 2020
Hu Min, Kang Guangbo, Cheng Xin, Wang Jiewen, Li Ruowei, Bai Zixuan, Yang Dong, Huang He
Abstract excerpt
Affinity is an important property of therapeutic antibodies, so improving affinity is critical to the biological activity and clinical efficacy. An anti-HIF-1α nanobody, VHH212, was screened via a native ribosome display library with a 26.6 nM of KD value was used as the parent. In this paper, a Venn-intersection of multi-algorithms screening (VIMAS) strategy for computer-aided binding affinity prediction was...
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