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Article

Femtomolar Fab binding affinities to a protein target by alternative CDR residue co-optimization strategies without phage or cell surface display.

mAbs - 1 Jan 2000

Votsmeier Christian, Plittersdorf Hanna, Hesse Oliver, Scheidig Andreas, Strerath Michael, Gritzan Uwe, Pellengahr Klaus, Scholz Peter, Eicker Andrea, Myszka David, Coco Wayne M, Haupts Ulrich

Abstract excerpt

In therapeutic or diagnostic antibody discovery, affinity maturation is frequently required to optimize binding properties. In some cases, achieving very high affinity is challenging using the display-based optimization technologies. Here we present an approach that begins with the creation and clonal, quantitative analysis of soluble Fab libraries with complete diversification in adjacent residue pairs...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 22531438
DOI: 10.4161/mabs.19981
PMCID: PMC3355236

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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