Improving antibody affinity through in vitro mutagenesis in complementarity determining regions

Abstract excerpt

High-affinity antibodies are widely used in diagnostics and for the treatment of human diseases. However, most antibodies are isolated from semi-synthetic libraries by phage display and do not possess in vivo affinity maturation, which is triggered by antigen immunization. It is therefore necessary to engineer the affinity of these antibodies by way of in vitro assaying. In this study, we optimized the affinity...

Topics

Join the communities discussing this publication.

• Glycosylation and Glycoproteins Research
• Monoclonal and Polyclonal Antibodies Research
• T-cell and B-cell Immunology