Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

In vitro nanobody discovery for integral membrane protein targets

24 Oct 2014

Abstract excerpt

Nanobodies (Nbs) or single-domain antibodies are among the smallest and most stable binder scaffolds known. In vitro display is a powerful antibody discovery technique used worldwide. We describe the first adaptation of in vitro mRNA/cDNA display for the rapid, automatable discovery of Nbs against desired targets, and use it to discover the first ever reported nanobody against the human full-length glucose...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Glycosylation and Glycoproteins Research
Share to Monoclonal and Polyclonal Antibodies Research
Share to RNA and protein synthesis mechanisms

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 25342225
DOI: 10.1038/srep06760

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

In vitro nanobody discovery for integral membrane protein targetsPMID 25342225Read ↗

Synthetic single domain antibodies for the conformational trapping of membrane proteinsSemantic proximity Read ↗

Advancements in nanobody generation: Integrating conventional, in silico, and machine learning approachesSemantic proximity Read ↗

Unique Binding and Stabilization Mechanisms Employed By and Engineered Into NanobodiesSemantic proximity Read ↗

Evolution of nanobodies specific for BCL11ASemantic proximity Read ↗

Structure-guided in vitro evolution of nanobodies targeting new viral variants.Semantic proximity Read ↗

A toolbox of nanobodies developed and validated for diverse neuroscience research applicationsSemantic proximity Read ↗

Ribosome Display: A Potent Display Technology used for Selecting and Evolving Specific Binders with Desired PropertiesSemantic proximity Read ↗

GPCR-targeting nanobodies: attractive research tools, diagnostics, and therapeuticsSemantic proximity Read ↗

Synthetic single domain antibodies for the conformational trapping of membrane proteinsSemantic proximity Read ↗

A cDNA Immunization Strategy to Generate Nanobodies against Membrane Proteins in Native ConformationSemantic proximity Read ↗

In Vitro Selection of Peptides and Proteins—Advantages of mRNA DisplaySemantic proximity Read ↗

Rapid generation of potent antibodies by autonomous hypermutation in yeastSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.