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Design of nanobody-based bispecific constructs by in silico affinity maturation and umbrella sampling simulations

16 Dec 2022

Abstract excerpt

Random mutagenesis is the natural opportunity for proteins to evolve and biotechnologically it has been exploited to create diversity and identify variants with improved characteristics in the mutant pools. Rational mutagenesis based on biophysical assumptions and supported by computational power has been proposed as a faster and more predictable strategy to reach the same aim. In this work we confirm that...

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PMID: 36659922
DOI: 10.1016/j.csbj.2022.12.021

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