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Article

Imaging protein-protein interactions inside living cells via interaction-dependent fluorophore ligation.

Journal of the American Chemical Society - 14 Dec 2011

Slavoff Sarah A, Liu Daniel S, Cohen Justin D, Ting Alice Y

Abstract excerpt

We report a new method, Interaction-Dependent PRobe Incorporation Mediated by Enzymes, or ID-PRIME, for imaging protein-protein interactions (PPIs) inside living cells. ID-PRIME utilizes a mutant of Escherichia coli lipoic acid ligase, LplA(W37V), which can catalyze the covalent ligation of a coumarin fluorophore onto a peptide recognition sequence called LAP1. The affinity between the ligase and LAP1 is tuned...

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PMID: 22098454
DOI: 10.1021/ja206435e
PMCID: PMC3547671

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