Article
Conversion of red fluorescent protein into a bright blue probe.
Chemistry & biology - 20 Oct 2008
Subach Oksana M, Gundorov Illia S, Yoshimura Masami, Subach Fedor V, Zhang Jinghang, Grüenwald David, Souslova Ekaterina A, Chudakov Dmitriy M, Verkhusha Vladislav V
Abstract excerpt
We used a red chromophore formation pathway, in which the anionic red chromophore is formed from the neutral blue intermediate, to suggest a rational design strategy to develop blue fluorescent proteins with a tyrosine-based chromophore. The strategy was applied to red fluorescent proteins of the different genetic backgrounds, such as TagRFP, mCherry, HcRed1, M355NA, and mKeima, which all were converted into blue...
Topics
- Amino Acid Sequence
- Amino Acid Substitution
- Color
- Fluorescence Resonance Energy Transfer
- Genes, Reporter
- HeLa Cells
- Humans
- Luminescent Proteins
- Molecular Probes
- Molecular Sequence Data