Article
DNB-based on-chip motif finding: A high-throughput method to profile different types of protein-DNA interactions.
Science advances - 1 Jul 2020
Li Zhuokun, Wang Xiaojue, Xu Dongyang, Zhang Dengwei, Wang Dan, Dai Xuechen, Wang Qi, Li Zhou, Gu Ying, Ouyang Wenjie, Zhao Shuchang, Huang Baoqian, Gong Jian, Zhao Jing, Chen Ao, Shen Yue, Dong Yuliang, Zhang Wenwei, Xu Xun, Xu Chongjun, Jiang Yuan
Abstract excerpt
Here, we report a sensitive DocMF system that uses next-generation sequencing chips to profile protein-DNA interactions. Using DocMF, we successfully identified a variety of endonuclease recognition sites and the protospacer adjacent motif (PAM) sequences of different CRISPR systems. DocMF can simultaneously screen both 5' and 3' PAMs with high coverage. For SpCas9, we found noncanonical 5'-NAG-3' (~5%) and...
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