Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Confirmation of Pig-a mutation in flow cytometry-identified CD48-deficient T-lymphocytes from F344 rats.

Mutagenesis - 1 May 2015

Revollo Javier, Pearce Mason G, Petibone Dayton M, Mittelstaedt Roberta A, Dobrovolsky Vasily N

Abstract excerpt

The Pig-a assay is used for monitoring somatic cell mutation in laboratory animals and humans. The assay detects haematopoietic cells deficient in glycosylphosphatidylinositol (GPI)-anchored protein surface markers using flow cytometry. However, given that synthesis of the protein markers (and the expression of their genes) is independent of the expression of the X-linked Pig-a gene and the function of its enzyme...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Antigens, CD
Share to Ethylnitrosourea

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Immunomagnetic Separation

Share to Mutagenesis

Share to Mutagenicity Tests

Share to Mutagens

Share to Rats, Inbred F344

Share to T-Lymphocytes

Source

Identifiers

PMID: 25820172
DOI: 10.1093/mutage/geu030

Genes

GPI

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Confirmation of Pig-a mutation in flow cytometry-identified CD48-deficient T-lymphocytes from F344 rats.PMID 25820172Read ↗

CD48-deficient T-lymphocytes from DMBA-treated rats have de novo mutations in the endogenous Pig-a gene.Semantic proximity Read ↗

Development of an in vivo gene mutation assay using the endogenous <i>Pig‐A</i> gene: I. Flow cytometric detection of CD59‐negative peripheral red blood cells and CD48‐negative spleen T‐cells from the ratSemantic proximity Read ↗

Analysis of mutations in the Pig-a gene of spleen T-cells from N-ethyl-N-nitrosourea-treated fisher 344 rats.Semantic proximity Read ↗

CD59-deficient bone marrow erythroid cells from rats treated with procarbazine and propyl-nitrosourea have mutations in the Pig-a gene.Semantic proximity Read ↗

In Vivo Rat T-Lymphocyte Pig-a Assay: Detection and Expansion of Cells Deficient in the GPI-Anchored CD48 Surface Marker for Analysis of Mutation in the Endogenous Pig-a Gene.Semantic proximity Read ↗

Detection of Pig-a mutant erythrocytes in the peripheral blood of rats and mice.Semantic proximity Read ↗

Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice.Semantic proximity Read ↗

Development of an in vivo gene mutation assay using the endogenous Pig-A gene: II. Selection of Pig-A mutant rat spleen T-cells with proaerolysin and sequencing Pig-A cDNA from the mutants.Semantic proximity Read ↗

Establishing a novel Pig-a gene mutation assay in L5178YTk+/- mouse lymphoma cells.Semantic proximity Read ↗

Spectrum of Pig-a mutations in T lymphocytes of rats treated with procarbazine.Semantic proximity Read ↗

Pig-a mutations in bone marrow erythroblasts of rats treated with 7,12-dimethyl-benz[a]anthracene.Semantic proximity Read ↗

The in vivo Pig-a gene mutation assay, a potential tool for regulatory safety assessment.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.