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Enhanced purification of cell-permeant Cre and germline transmission after transduction into mouse embryonic stem cells.

Genesis (New York, N.Y. : 2000) - 1 Aug 2007

Peitz Michael, Jäger Richard, Patsch Christoph, Jäger Andrea, Egert Angela, Schorle Hubert, Edenhofer Frank

Abstract excerpt

Continuous expression of Cre recombinase has the potential to yield toxic side effects in various cell types, thereby limiting applications of the Cre/loxP system for conditional mutagenesis. In this study, we investigate the potential of Cre protein transduction to overcome this limitation. COS-7, CV1-5B, and mouse embryonic stem (ES) cells treated with cell-permeant Cre (HTNCre) maintain a normal growth...

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PMID: 17661398
DOI: 10.1002/dvg.20321

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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