Article
Green-Sensitive, Long-Lived, Step-Functional Anion Channelrhodopsin-2 Variant as a High-Potential Neural Silencing Tool.
The journal of physical chemistry letters - 6 Aug 2020
Kojima Keiichi, Miyoshi Natsuki, Shibukawa Atsushi, Chowdhury Srikanta, Tsujimura Masaki, Noji Tomoyasu, Ishikita Hiroshi, Yamanaka Akihiro, Sudo Yuki
Abstract excerpt
Anion channelrhodopsin-2 (GtACR2) was identified from the alga Guillardia theta as a light-gated anion channel, providing a powerful neural silencing tool for optogenetics. To expand its molecular properties, we produced here GtACR2 variants by strategic mutations on the four residues around the retinal chromophore (i.e., R129, G152, P204, and C233). After the screening with the Escherichia coli expression...
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