Article
Reciprocal H3.3 gene editing identifies K27M and G34R mechanisms in pediatric glioma including NOTCH signaling.
Communications biology - 9 Jul 2020
Chen Kuang-Yui, Bush Kelly, Klein Rachel Herndon, Cervantes Vanessa, Lewis Nichole, Naqvi Aasim, Carcaboso Angel M, Lechpammer Mirna, Knoepfler Paul S
Abstract excerpt
Histone H3.3 mutations are a hallmark of pediatric gliomas, but their core oncogenic mechanisms are not well-defined. To identify major effectors, we used CRISPR-Cas9 to introduce H3.3K27M and G34R mutations into previously H3.3-wildtype brain cells, while in parallel reverting the mutations in glioma cells back to wildtype. ChIP-seq analysis broadly linked K27M to altered H3K27me3 activity including within...
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