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Article

Conjugative Transfer between Escherichia coli and Leptospira spp. as a New Genetic Tool

10 Nov 2007

Abstract excerpt

Our understanding of leptospiral pathogenesis, which remains poorly understood, depends on reliable genetic tools for functional analysis of genes in pathogenic strains. In this study, we report the first demonstration of conjugation between Escherichia coli and Leptospira spp. by using RP4 derivative conjugative plasmids. The DNA transfer described here was due to authentic conjugation, as shown by the...

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PMID: 17993560
DOI: 10.1128/aem.02172-07

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