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Article

Engineering caspase 7 as an affinity reagent to capture proteolytic products.

The FEBS journal - 1 Feb 2021

Razai Amir S, Snipas Scott J, Poreba Marcin, Fasci Domenico, Salvesen Guy S

Abstract excerpt

Many proteases recognize their substrates with high specificities, with this in mind, it should theoretically be possible to utilize the substrate binding cleft of a protease as a scaffold to engineer an affinity reagent. In this study, we sought to develop reagents that would differentiate between substrates and products of proteolysis, based on a caspase 7 scaffold. Firstly, we engineered a form of caspase 7...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32619291
DOI: 10.1111/febs.15467
PMCID: PMC7779697

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