Article
Measurement of Protease Activities Using Fluorogenic Substrates.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018
Santamaria Salvatore, Nagase Hideaki
Abstract excerpt
Matrix metalloproteinases and the related metalloproteases are implicated in cancer progression. They are endopeptidases that require several defined amino acid residues in both N-terminal and C-terminal sides of the scissile bond. Fluorogenic Förster resonance energy transfer (FRET) substrates that harbor a fluorophore and a quencher on opposite sides of the scissile bond are conveniently used to measure their...
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