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Article

Reprogramming Caspase-7 Specificity by Regio-Specific Mutations and Selection Provides Alternate Solutions for Substrate Recognition.

ACS chemical biology - 17 Jun 2016

Hill Maureen E, MacPherson Derek J, Wu Peng, Julien Olivier, Wells James A, Hardy Jeanne A

Abstract excerpt

The ability to routinely engineer protease specificity can allow us to better understand and modulate their biology for expanded therapeutic and industrial applications. Here, we report a new approach based on a caged green fluorescent protein (CA-GFP) reporter that allows for flow-cytometry-based selection in bacteria or other cell types enabling selection of intracellular protease specificity, regardless of the...

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Identifiers

PMID: 27032039
DOI: 10.1021/acschembio.5b00971
PMCID: PMC4912419

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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