Article
Enhancing the promiscuity of a member of the Caspase protease family by rational design.
Proteins - 1 Oct 2020
Öhlknecht Christoph, Petrov Drazen, Engele Petra, Kröß Christina, Sprenger Bernhard, Fischer Andreas, Lingg Nico, Schneider Rainer, Oostenbrink Chris
Abstract excerpt
The N-terminal cleavage of fusion tags to restore the native N-terminus of recombinant proteins is a challenging task and up to today, protocols need to be optimized for different proteins individually. Within this work, we present a novel protease that was designed in-silico to yield enhanced promiscuity toward different N-terminal amino acids. Two mutations in the active-site amino acids of human Caspase-2 were...
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